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PL Optics Olympus TIRF/HR objectives offer ultra-high NA up to 1.7 with flat-field apochromatic optics, optimized for TIRF and live-cell super-resolution imaging, featuring diverse magnifications and working distances for single-molecule and organelle studies.
Olympus Super-Resolution/TIRF Imaging Apochromat Objectives
(Olympus APON-TIRF / UAPON-TIRF Series)
Olympus TIRF/HR Objectives are engineered for Total Internal Reflection Fluorescence (TIRF) and ultra-high-resolution imaging, offering a numerical aperture (NA) up to 1.7 for exceptional light-collection and resolution. Their advanced optics and coatings minimize light loss and boost contrast—ideal for live-cell and organelle real-time super-resolution work. High NA also generates a potent evanescent field for high-contrast TIRF or super-resolution imaging, meeting modern microscopy’s exacting demands.
| Model | Magnification | NA | WD (mm) | FN | Cover-glass Thickness (mm) | Immersion | Spring | Fluorescence |
|---|---|---|---|---|---|---|---|---|
| UPLAPO60XOHR | 60X | 1.50 | 0.11 | 22 | 0.13–0.19 | Oil | None | U/BG/IR |
| UPLAPO100XOHR | 100X | 1.50 | 0.12 | 22 | 0.13–0.19 | Oil | None | U/BG/IR |
| APON100XHOTIRF | 100X | 1.70 | 0.08 | 22 | 0.15 | Special Oil | None | U/BG/IR |
| UAPON150XOTIRF | 150X | 1.45 | 0.08 | 22 | 0.13–0.19 | Oil | None | U/BG/IR |
UPLAPO60XOHR: Whole-cell TIRF imaging, live-cell real-time super-resolution, fine-structure (e.g., organelles) super-resolution
UPLAPO100XOHR: Live-cell super-resolution, sub-cellular high-resolution, single-molecule studies
APON100XHOTIRF: Single-molecule protein or vesicle tracking
UAPON150XOTIRF: Sub-cellular imaging (organelles, ER, vesicle transport)
